URINE PATHOGEN PANEL

Urinary Tract Infections (UTIs) account for 8.3 million doctor visits, 1 million emergency department (ED) visits, and 100,000 hospitalizations annually, with an estimated cost of $1 billion annually in the U.S.

The Problem:

The spread of fluoroquinolone resistant E. coli, extended spectrum beta-lactamase (ESBL), methicillin-resistant Staphylococcus aureus (MRSA), and vancomycin-resistant enterococci (VRE), among other emerging bacterial resistance factors contributes to increasing treatment cost and poor patient outcomes.

A New Tool in the Fight Against Antibiotic Resistance:

MicroPathID – Urine Pathogen Panel (UPP)Uses real-time PCR to identify and quantify 17 pathogenic bacteria and fungal species associated with infections of the urinary tract and detects up to 36 genes that are associated with antibiotic resistance across 10 drug classes.  Reflex urine culture and susceptibility results are also provided to help guide antibiotic selection and assist with antimicrobial stewardship.

Rapid PCR Detection
17 Pathogenic Bacteria
and Fungal Species


Acinetobacter baumannii
Citrobacter freundii
Enterobacter aerogenes
Enterobacter cloacae
Enterococcus faecalis
Enterococcus faecium
Escherichia coli
Klebsiella oxytoca
Klebsiella pneumoniae
Morganella morganii
Proteus mirabilis
Proteus vulgaris
Staphylococcus aureus
Pseudomonas aeruginosa
Staphylococcus saprophyticus
Streptococcus agalactiae
Candida albicans

Rapid PCR Detection
36 Gene Antibiotic
Resistance Panel
~10 Drug Classes


Available as a reflex on a positive pathogen.

Aminoglycoside
AmpC beta lactamase
Extended Spectrum Betalactamase
Carbapenem
Fluoroquinolone
Macrolides, lincosamides, streptogramins
Methicillin
Tetracycline
Trimethoprim/Sulfamethoxazole
Vancomycin

Confirmatory Culture
MALDI-TOF ID
and Susceptibility


Available as a reflex on a positive pathogen.

Identification
CFU/mL
Antibiotic Susceptibility 
MIC

Sexually Transmitted Infection Panel

MicroPathID-UPP Panel also offers tests for the 4 common STI pathogens, including Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, and Mycoplasma genitalium.

Clinical Advantages:  MicroPathID – UPP Panel

  • Rapid PCR results on pathogen identification
    • 24 – 48 hours from specimen receipt
    • More sensitive than conventional culture
    • Can detect polymicrobial infections
    • Unaffected by concurrent antibiotic use
  • Phenotypic characterization combined with genotypic information for improved outcomes
    • Reflex culture and susceptibility on a positive pathogen
    • Reflex antibiotic resistance genotyping on a positive pathogen

The Process

The Urine Pathogen Panel is performed from a clean catch urine specimen.

Day 1
Collect a clean catch urine sample.

Day 2
Specimen arrives at the laboratory – PCR testing begins for pathogen identification.

  • Negative results are finalized and released.
  • Cultures begin.
  • Antibiotic Resistance Gene Panels are added as a reflex on specimens testing positive by molecular methods for one or more of the 17 pathogens.

Day 3 – 4
Receive PCR results of the Urine Pathogen Panel along with antibiotic resistance genotyping and confirmatory culture and susceptibility results.

Day 4
Informed antibiotic selection

References:
1 Foxman B. Epidemiology of urinary tract infections: Incidence, morbidity, and economic costs. Am J Med.2002;113(suppl 1A):5S-13S.

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