Antibiotic-resistant bacteria represent an increasing concern in wound infections.
The Problem:
Chronic wounds caused by vascular insufficiency often show multiple bacterial strains which are resistant to common antibacterials. The recent spread of fluoroquinolone resistant E. coli, extended spectrum beta-lactamase (ESBL), methicillin-resistant Staphylococcus aureus (MRSA), and vancomycin-resistant enterococci (VRE), among other emerging bacterial resistance factors contributes to increasing treatment cost and poor patient outcomes.
A New Tool in Wound Care and the Fight Against Antimicrobial Resistance:
MicroPathID – Wound Pathogen Panel (WPP) – Uses real-time PCR to identify and quantify 17 pathogenic bacteria and fungal species associated with wound infections and detects of up to 36 genes that confer antibiotic resistance across 10 drug classes. Confirmatory wound culture and susceptibility results are also provided to help guide antibiotic selection and assist with antimicrobial stewardship.
Rapid PCR Detection
17 Pathogenic Bacteria
and Fungal Species
Acinetobacter baumannii
Citrobacter freundii
Enterobacter aerogenes
Enterobacter cloacae
Enterococcus faecalis
Enterococcus faecium
Escherichia coli
Klebsiella oxytoca
Klebsiella pneumoniae
Morganella morganii
Proteus mirabilis
Proteus vulgaris
Staphylococcus aureus
Pseudomonas aeruginosa
Staphylococcus saprophyticus
Streptococcus agalactiae
Candida albicans
Rapid PCR Detection
36 Gene Antibiotic
Resistance Panel
~10 Drug Classes
Aminoglycoside
AmpC beta lactamase
Extended Spectrum Betalactamase
Carbapenem
Fluoroquinolone
Macrolides, lincosamides, streptogramins
Methicillin
Tetracycline
Trimethoprim/Sulfamethoxazole
Vancomycin
Confirmatory Culture
MALDI-TOF ID
and Susceptibility
Identification
CFU/mL
Antibiotic Susceptibility
MIC
Conventional Cultures:
- Are unable to produce growth in certain pathogens
- Require 48-72 hours for results
Clinical Advantages: MicroPathID – WPP Panel
- Rapid PCR results on pathogen identification and antibiotic resistance
- 12 – 24 hours from specimen receipt
- Improved Sensitivity
- 3-5X more sensitive than conventional culture
- Can detect polymicrobial infections
- Unaffected by concurrent antibiotic use
- Culture confirmation and susceptibility
- Phenotypic characterization combined with genotypic information for improved outcomes
The Process
The Wound Pathogen Panel is performed by by Poplar Healthcare from a swab specimen collected in a BD ESwab media (liquid Amies).
Day 1
Collect wound swab sample
Day 2
Specimen arrives at Poplar Healthcare – PCR testing and cultures begin
Day 2-3
Receive PCR results of 17 pathogens and Antibiotic Resistance Genes
Day 4
Receive confirmatory culture and susceptibility results
Day 4
Informed antibiotic selection
For more information about Poplar Healthcare and D-PATH click the logos below to visit their full sites.
